High-throughput gene expression analysis at the level of single proteins using a microfluidic turbidostat and automated cell tracking
نویسندگان
چکیده
منابع مشابه
High-throughput gene expression analysis at the level of single proteins using a microfluidic turbidostat and automated cell tracking.
We have developed a method combining microfluidics, time-lapsed single-molecule microscopy and automated image analysis allowing for the observation of an excess of 3000 complete cell cycles of exponentially growing Escherichia coli cells per experiment. The method makes it possible to analyse the rate of gene expression at the level of single proteins over the bacterial cell cycle. We also dem...
متن کاملHi-throughput gene expression analysis at the level of single proteins using a microfluidic turbidostat and automated cell tracking
We have developed a method combining microfluidics, time-lapsed single-molecule microscopy and automated image analysis allowing for the observation of an excess of 3000 complete cell cycles of exponentially growing Escherichia coli cells per experiment. The method makes it possible to analyze the rate of gene expression at the level of single proteins over the bacterial cell cycle. We also dem...
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ژورنال
عنوان ژورنال: Philosophical Transactions of the Royal Society B: Biological Sciences
سال: 2013
ISSN: 0962-8436,1471-2970
DOI: 10.1098/rstb.2012.0025